Description |
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TaqDNAPolymeraseistheenzymefromtheclonedThermusaquaticusexpressedinE.coli.ThisunmodifiedenzymereplicatesDNAat74°Candexhibitsahalf-lifeof40minutesat95°C.TheenzymecatalyzesthepolymerizationofnucleotidesintoduplexDNAinthe5"→3"directioninthepresenceofmagnesiumandalsopossessesa5"→3"exonucleaseactivity. TaqDNAPolymeraseisrecommendedforuseinPCRbutisnotrecommendedforuseinDNAsequencingreactions. |
Specification |
•Ultrapure,Thermostablerecombinantenzyme •Highlythermostable:10minat97°C,60minat94°C •5"→3"exonucleaseactivity •ExtraAadditionactivity •Amplificationrange: -LamBDaDNA:upto6Kb(Max.12Kb) -HumangenomicDNA:upto2Kb(Max.4Kb) •CompositionofTaqpolymeraseandTaqPCRbuffercontainingMgCl2 •NoNuclease,RNase,Proteasecontamination |
Components |
•TaqDNAPolymeraseI,500U •10XTaqPCRBufferI(withMgCl2) •dNTPMix(2.5mMeach) •10XLoADIngBuffer •NucleaseFreeWater •4XMagicBuffer(forhighGCcontent) |
Citation |
■Thegene–dietinteraction,LPLPvuIIandHindIIIandcarbohydrate,onthecriteriaofmetabolicsyndrome:KMSRI-SeoulStudy YKim,MLee,YLim,YJang,HKPark,YLee Nutrition,2013 ■SupplementationofKoreanfermentedsoypastedoenjangreducesvisceralfatinoverweightsubjectswithmutantuncouplingprotein-1allele MLee,SChae,YCha, NutritionResearch,2012 ■TheassociationoflipoproteinlipasePvuIIpolymorphismandniacinintakeintheprevalenceofmetabolicsyndrome:aKMSRI-Seoulstudy EShin,NYPark,YJang,HOh,JJeong,YLim Genes&Nutrition,2011 ■CharacterizationoftheChaetocerossalsugineumnuclearinclusionviruscoatproteingene YJPark,SEJung,YTomaru,WBChoi,YHKim,HMizumoto,KNagasakiandTJChoi VirusResearch,Volume142,Issues1-2,June(2009)Pages127-133 ■NovelMolecularMechanismforActinomycinDActivityasanOncogenicPromoterG-QuadruplexBinder Hyun-JinKangandHyun-JuPark Biochemistry,2009,48(31),pp7392-7398 ■StromalCell-DerivedFactor-1PromotesMyelomaCellGrowthinBothAutocrineandParacrineManners Seong-WooKim,Ha-YonKim,Hyo-JinLee,M.D.,Hwan-JungYun,M.D.,SamyongKim,M.D.andDeog-YeonJo,M.D. KoreanJHematol.2008Sep;43(3):127-137 ■PossIBLeassociationbetweenSEROtonintransporterpromoterregionpolymorphismandimpulsivityinKoreans. JHLee,HTKim,DSHyun PsychiatryResearch,Volume118,Issue1,Pages19-24,2003 |
KOMA Biotechnology是1994年成立于首尔的一家综合性生物研究工具生产企业,其名称来源于韩国古代传说,意为韩国民族来源的一位英雄,同时KOMA也有"感谢你"的意思。 KOMA企业在韩国确实有其名称来源的意味,20年间,KOMA共开发出5大类近3000种产品,横跨免疫学,分子生物学,实验仪器三大领域,成为韩国生物技术企业的领先者和进军国际市场的急先锋。目前要提供各式分子生物学、免疫学、细胞生物学、蛋白质组学和基因研究相关试剂。产品包括蛋白电泳系统和预制胶、PCR扩增试剂、转染试剂、直接PCR、核酸纯化试剂以及 ELISA试剂盒等。除一般细胞因子 (Cytokine)及免疫球蛋白 (Immunoglobulin) ELISA 试剂盒外,也提供 ELISA core Kit (Coating Antibody) 及 ELISA Combo Kit 满足客户不同的需求。