Description |
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EzWay™TotalRNAIsolationKitisdesignedforextractionoftotalRNAfromanimaltissueandculturedcells.Somespeciallymodifiedprotocolsaredevelopedforothersamples,suchasbacteriaandyeast.Thismethodfirstlysescellsbyusingachaotropicsalt,thenbindsRNAtosilica-basedmembranes,washesRNAwithethanol-containedwashbufferandthenelutespurifiedRNAbyRNase-freeddH2O.Ittakes30minforanentireprocedure,andthepurifiedRNAisreadyforRT-PCR,northernblotting,primerextensionandCDNAlibraryconstruction. |
Specification |
•BindingCapacity:45ug •ColumnCapacity:800ul •RecoveryRate:85-95% •Yield:12ul •A260/A280:2 |
Components |
•LysisBuffer •WashBufferA •WashBufferB •DEPC-treatedwater •RNA-prepColumn •2mlCollectionTube •ElutionTube |
Principle |
RNAreleasedfromtissuebychaotropicsalt-containinglysisbufferisboundtosilica-basedmembranes.ThepurifiedRNAonthemembraneiselutedwithRNase-freeddH2O. |
Applications |
•RT-PCR •Northernblotting •Primerextension •cDNAlibraryconstruction |
Citation |
■TheprophylacticeffectofprobioticBacilluspolyfermenticusKU3againstcancercells NKLee,SHSon,EBJeon,GHJung,JYLee,HDPaik JournalofFunctionalFoods,2015 ■ProbioticpotentialofLactobacillusstrainswithanti-allergiceffectsfromkimchiforyogurtstarters NKLee,SYKim,KJHan,SJEom,HDPaik LWT-FoodScienceandTechnology,2014 |
KOMA Biotechnology是1994年成立于首尔的一家综合性生物研究工具生产企业,其名称来源于韩国古代传说,意为韩国民族来源的一位英雄,同时KOMA也有"感谢你"的意思。 KOMA企业在韩国确实有其名称来源的意味,20年间,KOMA共开发出5大类近3000种产品,横跨免疫学,分子生物学,实验仪器三大领域,成为韩国生物技术企业的领先者和进军国际市场的急先锋。目前要提供各式分子生物学、免疫学、细胞生物学、蛋白质组学和基因研究相关试剂。产品包括蛋白电泳系统和预制胶、PCR扩增试剂、转染试剂、直接PCR、核酸纯化试剂以及 ELISA试剂盒等。除一般细胞因子 (Cytokine)及免疫球蛋白 (Immunoglobulin) ELISA 试剂盒外,也提供 ELISA core Kit (Coating Antibody) 及 ELISA Combo Kit 满足客户不同的需求。