Description |
---|
EzWay™GelExtractionKitisdesignedforrapidpurificationofDNAfromstandardagarosegels.Itprovidesafastandsimplemethodusingadvancedsilica-basedmembranetechnologyforrapidandefficientpurificationof100bp-10kbDNAfragments.DNAadsorbstothesilica-membraneinthepresenceofhighsaltwhilecontaminantspassthroughthecolumn.Impuritiesareefficientlywashedaway,andthepureDNAiselutedwithElutionBufferorwater.ThepurifiedDNAissuitableforavarietyofapplicationssuchasfluorescentDNAsequencing,transformation,restrictionmapping,cloning,andlabeling. |
Specification |
•SampleAmount:upto200mgofagarosegel •Recovery:70%-85% •Operationtime:within20min •Elutionvolume:40ul |
Components |
•DNA-prepColumn •CollectionTube •ElutionTube •ExtractionBuffer •WashBuffer •ElutionBuffer |
Principle |
AfteradditionofExtractionBuffertoagarosegelslicesampleandheatingat50-55°Ctomeltgel,DNAisboundtoasilicagelmembraneinthepresenceofhighconcentrationofchaotropicsalt.ThepurifiedDNAproductonthemembraneiselutedinelutionbuffer. |
Applications |
•FluorescentDNAsequencing •Ligation •Transformation •PCR •Micro-injection •DNAmicroarry-basedanalysis •Restrictionmapping •Labelingetc. |
Citation |
■MolecularandPhysiologicalMechanismsofHeavyMetalToleranceinAtriplexhalimus REl-Bakatoushi,AMAlframawy,ATammam InternationalJournalofPhytoremediation2015 ■FurtherMolecularCharacterizationandEffectonHostPhotosyntheticPigmentsandCarbohydratePoolsofanEgyptianIsolateofTBSV FAFattouh,ASAli,RMFathy JPlantPatholMicrob,2015 ■MolecularphylogenyevidenceofaltitudinaldistributionandhABItatadaptationinKoreanEphemeraspecies(Ephemeroptera:Ephemeridae) JMHwang,TJYoon,KISuh,YJBae EntomologicalResearch,2013 ■Identificationandcharacterizationofup-regulatedgenesinthehalophyteLimoniastrummonopetalum(L.)Boissgrownundercrudeoilpollution REl-Bakatoushi JournalofGeneticEngineeringandBiotechnology,2011 |
KOMA Biotechnology是1994年成立于首尔的一家综合性生物研究工具生产企业,其名称来源于韩国古代传说,意为韩国民族来源的一位英雄,同时KOMA也有"感谢你"的意思。 KOMA企业在韩国确实有其名称来源的意味,20年间,KOMA共开发出5大类近3000种产品,横跨免疫学,分子生物学,实验仪器三大领域,成为韩国生物技术企业的领先者和进军国际市场的急先锋。目前要提供各式分子生物学、免疫学、细胞生物学、蛋白质组学和基因研究相关试剂。产品包括蛋白电泳系统和预制胶、PCR扩增试剂、转染试剂、直接PCR、核酸纯化试剂以及 ELISA试剂盒等。除一般细胞因子 (Cytokine)及免疫球蛋白 (Immunoglobulin) ELISA 试剂盒外,也提供 ELISA core Kit (Coating Antibody) 及 ELISA Combo Kit 满足客户不同的需求。