Description |
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AmajorlimitationofPolymeraseChainReaction(PCR)-basedresearchanddiagnosticandforensictestsistheinhibitionofDNApolymerasesbymanysubstanceslikeasanticoagulants(e.g.,heparin,EDTA,sodiumcitrate)andhemegroup,proteins,polysaccharides,celldebris,andchargedinorganiccompounds,etc.Therefore,DNAisusuallyextractedfromspecimenaccordingtophenol-chloroformmethodorbyanalternative‘silica-membranefilter’method.However,thisistimeconsumingandlaborintensiveandthereisalsoariskofintroducingcontaminationduringthepreparation. EzWayDirectFPCRallowsdirectPCRfrombuccalorbloodFTAdisc,bloodorhairlysatewithoutanyDNApurificationstepsjustbyimmersingtheFTApunchorpipettinglysateintoPCRmixture.EzWayDirectFBuffercontainstheproperamountsofbuffer,MgCl2,dNTPsandthecomponentsthatfacilitatedirectPCRamplification,buthotstartenzymeissuppliedwithseparatetubefortheextendedactivity |
Specification |
•DirectPCRfrombuccalorbloodFTAdisc,bloodorhairlysatewithoutanyDNApurificationstepsjustbyimmersingtheFTApunchorpipettinglysateintoPCRmixture |
Components |
•EzWayDirectFBufferMix100T(25uLPCR) •HotTaq-fx(5units/uL) •25xInhibitorcocktail |
Platform |
•AppliedBiosystems310GeneticAnalyzerfilledwithPOP-4&36cmcapillaries •ABIPRISM®3100/3100-AvantGeneticAnalyzerfilledwithPOP-4&36cmcapillaries •AppliedBiosystems3130/3130xlGeneticAnalyzerfilledwithPOP-4&36cmcapillaries •GeneAmp®PCRSystem9600/9700/Veriti |
Applications |
Humanidentificationwithaforensicsample |
Citation |
KOMA Biotechnology是1994年成立于首尔的一家综合性生物研究工具生产企业,其名称来源于韩国古代传说,意为韩国民族来源的一位英雄,同时KOMA也有"感谢你"的意思。 KOMA企业在韩国确实有其名称来源的意味,20年间,KOMA共开发出5大类近3000种产品,横跨免疫学,分子生物学,实验仪器三大领域,成为韩国生物技术企业的领先者和进军国际市场的急先锋。目前要提供各式分子生物学、免疫学、细胞生物学、蛋白质组学和基因研究相关试剂。产品包括蛋白电泳系统和预制胶、PCR扩增试剂、转染试剂、直接PCR、核酸纯化试剂以及 ELISA试剂盒等。除一般细胞因子 (Cytokine)及免疫球蛋白 (Immunoglobulin) ELISA 试剂盒外,也提供 ELISA core Kit (Coating Antibody) 及 ELISA Combo Kit 满足客户不同的需求。