Description |
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EzWay™AnnexinV-FITCApoptosisKitcanidentifyapoptosisatanearlierstagethanDNA-basedassayssuchasTUNEL.AnnexinVisacalcium-dependantphospholipidbindingproteinthathasahighaffinityforphosphatidylserine(PS),aplasmamembranephsopholipid.Innon-apoptoticcells,mostPSarelocalizedattheinnerlayeroftheplasmamembrane,butafterinducingapoptosis,PSareexposedtotheextracellularenvironment,andthenAnnexinVbindstoPS.CellsthatstainpositiveforAnnexinV-FITC(green)andnegativeforPIareundergoingapoptosis.CellsthatstainpositiveforbothAnnexinV-FITC(green)andPI(red)areeitherintheendstageofapoptosis,areundergoingnecrosis,orarealreadydead.CellsthatstainnegativeforbothAnnexinV-FITCandPIarealiveandnotundergoingmeasurableapoptosis. |
Specification |
•UtilizesFITC-conjugatedAnnexinVproteinfordetectionofcellsundergoingapoptosis •AnnexinVFITCbindstothemembranesofapoptoticcells •Detectionbyflowcytometryorfluorescencemicroscopy |
Components |
•AnnexinV-FITC •5XBindingbuffer •PropidiumIodide(PI) |
Citation |
■Trifloxystrobininducestumornecrosisfactor-relatedapoptosis-inducingligand(TRAIL)-mediatedapoptosisinHaCaT,humankeratinocytecells YJang,AYLee,SHChang,SHJeong,KHPark DrugandChemicalToxicology,2016 ■Nicotine-InducedApoptosisinHumanRenalProximalTubularEpithelialCells CSKim,JSChoi,SYJoo,EHBae,SKMa,JULee PLoSOne,2016 ■CytotoxicCompoundsfromJuglanssinensisDodeDisplayAnti-ProliferativeActivitybyInducingApoptosisinHumanCancerCells YJLee,JCui,JLee,ARHan,EBLee,HHJang Molecules,2016 ■5-lipoxygenasemediatesdocosahexaenoylethanolamideandN-arachidonoyl-L-alanine-inducedreactiveoxygenspeciesproductionandinhibitionofproliferationofheadandnecksquamouscellcarcinomacells SWPark,JHHah,SMOh,WJJeong,MWSung BMCCance ■ChlorpyrifosinducesNLRP3inflammasomeandpyroptosis/apoptosisviamitochondrialoxidativestressinhumankeratinocyteHaCaTcells YJang,AYLee,SHJeong,KHPark,MKPaik,NJCho Toxicology,2015 ■Cold-inducIBLeRNA-bindingprotein,CIRP,inhibitsDNAdamage-inducedapoptosisbyregulatingp53 HNLee,SMAhn,HHJang BiochemicalandBiophysicalResearchCommunications2015 ■SaururuschinensisBaillinducesapoptosisthroughendoplasmicreticulumstressinHepG2hepatocellularcarcinomacells AYLee,YAHan,JEKim,SHHong,EJPark,MHCho FoodandChemicalToxicology2015 ■DielectricBarrierDischargePlasmaEfficientlyDeliversanApoptoticResponseinHumanMonocyticLymphoma NKaushik,NKumar,CHKim,NKKaushik,EHChoi PlasmaProcessesandPolymers,2014 ■CXCR2anditsrelatedligandsplayanovelroleinsupportingthepluripotencyandproliferationofhumanpluripotentstemcells JHJung,SJLee,JHKim,SHLee,HJSung,JAn StemCellsandDevelopment,2014 ■AlteredAntioxidantSystemStimulatesDielectricBarrierDischargePlasma-InducedCellDeathforSolidTumorCellTreatment NKKaushik,NKaushik,DPark,EHChoi PLOSONE,2014 ■EpigeneticmodificationofNrf2in5-fluorouracil-resistantcoloncancercells:involvementofTET-dependentDNAdemethylation KAKang,MJPiao,KCKim,HKKang,WYChang CellDeath&Disease,2014 ■InhibitionofC2C12myotubeatrophybyanovelHSP70inducer,celastrol,viaactivationofAkt1andERK1/2pathways TGwag,KPark,EKim,CSon,JPark,TNikawa,IChoi ArchivesofBiochemistryandBiophysics2013 ■AmurensinGinducesautophagyandattenuatescellulartoxicitiesinarotenonemodelofParkinson"sdisease HRyu,WKOh,ISJang,JPark BiochemicalandBiophysicalResearchCommunications2013 ■Macrophage-stimulatingproteinattenuateshydrogenperoxide-inducedapoptosisinhumanrenalHK-2cells KELee,EYKim,CSKim,JSChoi,EHBae,SKMa EuropeanJournalofPharmacology2013 ■Macrophage-stimulatingproteinattenuatesgentamicin-inducedinflammationandapoptosisinhumanrenalproximaltubularepithelialcells KELee,EYKim,CSKim,JSChoi,EHBae,SKMa BiochemicalandBiophysicalResearchCommunications2013 ■AMAPkinasepathwayisimplicatedinthepseudohyphalinductionbyhydrogenperoxideinCandicaalbicans KSrinivasa,JKim,SYee,WKim,WJChoi MoleculesandCells,2012 |
KOMA Biotechnology是1994年成立于首尔的一家综合性生物研究工具生产企业,其名称来源于韩国古代传说,意为韩国民族来源的一位英雄,同时KOMA也有"感谢你"的意思。 KOMA企业在韩国确实有其名称来源的意味,20年间,KOMA共开发出5大类近3000种产品,横跨免疫学,分子生物学,实验仪器三大领域,成为韩国生物技术企业的领先者和进军国际市场的急先锋。目前要提供各式分子生物学、免疫学、细胞生物学、蛋白质组学和基因研究相关试剂。产品包括蛋白电泳系统和预制胶、PCR扩增试剂、转染试剂、直接PCR、核酸纯化试剂以及 ELISA试剂盒等。除一般细胞因子 (Cytokine)及免疫球蛋白 (Immunoglobulin) ELISA 试剂盒外,也提供 ELISA core Kit (Coating Antibody) 及 ELISA Combo Kit 满足客户不同的需求。