Koma BioTech/Pfu DNA Polymerase Kit/K0566001/250U,Koma BioTech,,Koma BioTech,komabiotech,KOMA Biotechnology,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Koma BioTech/Pfu DNA Polymerase Kit/K0566001/250U

产品编号：K0566001

市场价：¥0.00

场地：美国(厂家直采)

产品分类：生化试剂>缓冲液>酸碱缓冲液>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Koma BioTech

公司：Koma BioTech

公司分类：

商品介绍

Description
Pfu DNA polymerase isolated from Pyrococcus furiosus, a proofreading DNA polymerase due to 3'→5'exonuclease activity exhibits the lowest error rate of any thermostable DNA polymerase studied. This feature makes Pfu DNA polymerase the ideal choice for a variety of techniques requiring high fidelity DNA synthesis by PCR such as cloning, gene expression, and site-directed mutagenesis. Pfu DNA polymerase is a highly thermostable enzyme, retaining 94-99% of its polymerase activity after 1 hour at 95°C. To amplify GC-rich regions, denaturation temperature up to 98C can be used to with Pfu DNA polymerase. Pfu DNA polymerase is devoid of terminal deoxynucleotidyltrasferase (TdT) activity, which is characterized by the addition of nontemplate-directed nucleotide(s) at the 3'-end of PCR-generated fragments. Therefore, it generates blunt-ended PCR products. This kit contains all optimized reagents for PCR reaction.
Specification
• High PCR fidelity with improved yield • Highly thermostable: active more than 4 hrs at 95°C • Source: E.coli, recombinant gene from Pyrococcus furiosus • Lowest error rate • 3'→5' exonuclease activity • No extra A addition activity: Generate blunt-ended PCR products • Amplification range: - Lambda DNA: up to 60Kb - Human genomic DNA: up to 2Kb • Composition of all optimized reagents for PCR reaction (Pfu DNA Polymerase, Pfu PCR buffer containing MgSO4, dNTP mix, loading dye, nuclease free water and Magic buffer) in separate tubes • A supply of enhancer (Magic buffer) for GC rich template or secondary structures
Components
• Pfu DNA Polymerase • 10X Pfu PCR Buffer • 10X dNTP Mix (2.5mM each) • 10X Loading Buffer • Nuclease Free Water • 4X Magic Buffer
Citation

品牌介绍

KOMA Biotechnology是1994年成立于首尔的一家综合性生物研究工具生产企业，其名称来源于韩国古代传说，意为韩国民族来源的一位英雄，同时KOMA也有"感谢你"的意思。 KOMA企业在韩国确实有其名称来源的意味，20年间，KOMA共开发出5大类近3000种产品，横跨免疫学，分子生物学，实验仪器三大领域，成为韩国生物技术企业的领先者和进军国际市场的急先锋。目前要提供各式分子生物学、免疫学、细胞生物学、蛋白质组学和基因研究相关试剂。产品包括蛋白电泳系统和预制胶、PCR扩增试剂、转染试剂、直接PCR、核酸纯化试剂以及 ELISA试剂盒等。除一般细胞因子 (Cytokine)及免疫球蛋白 (Immunoglobulin) ELISA 试剂盒外，也提供 ELISA core Kit (Coating Antibody) 及 ELISA Combo Kit 满足客户不同的需求。